Related Experiment Video
Updated: Aug 18, 2026

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
Published on: February 21, 2018
[Granulocyte-macrophage colony-stimulating factor and erythropoietin induce tyrosine phosphorylation and activation
1Institute of Hematology, People's Hospital, Beijing Medical University, Beijing 100044.
Objective:
In order to identify the tyrosine kinase (s) that may associate with cell proliferation.
Methods:
GM-CSF/Epo responsive cell UT-7 and Epo responsive cell UT-7/Epo were used for study of tyrosine phosphorylation.
Results:
The addition of GM CSF and/or Epo to the responsive cells induced rapid tyrosine phosphorylations of 145000, 130000, 80000 and 40000 cellular proteins. The tyrosine phosphorylated 130000 protein was identified as JAK2, a non receptor tyrosine kinase. GM CSF and Epo also activated its tyrosine kinase activity. Tyrosine phosphorylation and activation of JAK2 only occurred in GM-CSF and/or Epo-responsive cells.
Conclusion:
JAK2 signal transduction pathway may play an important role in regulating the proliferation of hematopoietic cells induced by GM-CSF and Epo.
More Related Videos
Related Concept Videos
Mitogens and the Cell Cycle
Differentiation of Common Myeloid Progenitor Cells
Regulation of Hematopoietic Stem Cells
MAPK Signaling Cascades
The JAK-STAT Signaling Pathway
Role of Hematopoietic Growth Factors
Thrombopoietin (TPO), mainly released by the liver,...

