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Antimicrobial activity of UMFix tissue fixative.

T J Cleary1, A R Morales, M Nadji

  • 1Department of Pathology, Holtz Center, University of Miami School of Medicine, Miami, FL 33136, USA. tcleary@med.miami.edu

Journal of Clinical Pathology
|December 30, 2004
PubMed
Summary

UMFix, an alcohol-based tissue fixative, rapidly kills most microorganisms including bacteria, yeasts, and viruses. However, it is ineffective against bacterial spores, unlike neutral buffered formalin.

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Area of Science:

  • Microbiology
  • Biochemistry

Background:

  • Tissue fixatives are crucial for preserving biological samples.
  • Alcohol-based fixatives offer an alternative to traditional formalin-based solutions.
  • Understanding the antimicrobial properties of fixatives is essential for laboratory safety and sample integrity.

Purpose of the Study:

  • To evaluate the antimicrobial efficacy of UMFix, an alcohol-based tissue fixative.
  • To compare the antimicrobial effects of UMFix with 10% neutral buffered formalin.

Main Methods:

  • Standard microbiological methods were employed to assess antimicrobial activity.
  • Microorganism colony counts were determined after exposure to UMFix and formalin.
  • Comparative analysis of killing rates against various microbial types.

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Main Results:

  • UMFix demonstrated rapid microbicidal activity against vegetative bacteria, yeasts, molds, and viruses.
  • Bacterial spores exhibited resistance to UMFix.
  • 10% neutral buffered formalin effectively eliminated all tested microorganisms.

Conclusions:

  • UMFix is effective against a broad spectrum of microorganisms, excluding bacterial spores.
  • The methanol component in UMFix is responsible for its antimicrobial action.
  • Polyethylene glycol alone does not possess significant antimicrobial properties.