Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methylation-specific PCR unraveled.

Sarah Derks1, Marjolein H F M Lentjes, Debby M E I Hellebrekers

  • 1Research Institute GROW, Department of Pathology, University Maastricht, PO Box 616, 6200 MD Maastricht, The Netherlands.

Cellular Oncology : the Official Journal of the International Society for Cellular Oncology
|December 30, 2004
PubMed
Summary

Methylation-specific PCR (MSP) analyzes DNA methylation status by converting unmethylated cytosines to uracil and detecting sequence differences. This review covers MSP methods, variants, and clinical applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical Long-Read Genome Sequencing for Rare-Disease Diagnostics.

The New England journal of medicine·2026
Same author

Leveraging Commercially Available Protein Assays as Biomarkers for Lung Cancer.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026
Same author

Treatment interval in curative treatment of colon cancer and its impact on (cancer-free) survival in high-risk and non-high-risk patients.

Journal of geriatric oncology·2026
Same author

Genetic counselling implementation in dilated cardiomyopathy.

European heart journal·2026
Same author

Lnc5q21.2, a novel long intergenic RNA, sensitizes colorectal cancer cells to ATR inhibitor by activating Wnt pathway.

Journal of translational internal medicine·2025
Same author

Sex Differences in Prognosis of Patients With Genetic Dilated Cardiomyopathy.

Circulation. Heart failure·2025

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • DNA methylation is a key epigenetic modification regulating gene expression.
  • Aberrant DNA methylation patterns are implicated in various diseases, including cancer.
  • Accurate methods are needed to analyze DNA methylation status.

Purpose of the Study:

  • To review the principles and critical parameters of Methylation-specific PCR (MSP).
  • To provide an overview of existing MSP variants.
  • To discuss the clinical applications of MSP in disease diagnosis and research.

Main Methods:

  • Sodium bisulfite conversion of DNA to alter unmethylated cytosines.
  • Polymerase Chain Reaction (PCR) using methylation-sensitive and insensitive primers.

Related Experiment Videos

  • Analysis of sequence differences to determine DNA methylation status.

    • Main Results:

    • MSP is a cost-effective and rapid technique for analyzing CpG island methylation.
    • Different MSP variants offer flexibility for various research needs.
    • MSP has demonstrated utility in clinical diagnostics and biomarker discovery.

    Conclusions:

    • MSP is a valuable tool for studying DNA methylation.
    • Understanding MSP parameters is crucial for reliable results.
    • MSP continues to be relevant in epigenetic research and clinical practice.