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Diminished expression of CD19 in B-cell lymphomas
Wei Yang1, Neeta Agrawal, Jigar Patel
1University Hospitals of Cleveland and Case Western Reserve University, Cleveland, Ohio, USA.
Cytometry. Part B, Clinical Cytometry
|December 30, 2004
Summary
Diminished CD19 expression is common in B-cell lymphomas, especially follicular lymphoma. This finding aids in identifying lymphoma cells in complex samples like bone marrow.
Area of Science:
- Hematology
- Immunophenotyping
- Oncology
Background:
- CD19 is a key B-cell marker, but its expression levels in various B-cell lymphomas are not fully understood.
- Low-level CD19 expression can complicate lymphoma diagnosis.
Purpose of the Study:
- To investigate the frequency and patterns of decreased CD19 expression across different B-cell lymphoma subtypes.
- To assess the diagnostic utility of CD19 dim expression in B-cell malignancies.
Main Methods:
- Retrospective review of 349 B-cell lymphoma cases using flow cytometry.
- Analysis of CD19 expression levels compared to normal B lymphocytes.
- Correlation of flow cytometry findings with morphologic diagnoses.
Main Results:
- 36% of cases exhibited decreased CD19 expression.
- Follicular lymphoma showed the highest frequency (79%) of CD19 dim expression.
- CD19 dim expression was observed in small lymphocytic lymphoma/chronic lymphocytic leukemia, mantle cell lymphoma, diffuse large B-cell lymphoma, and marginal zone lymphoma, but not in Burkitt lymphoma or hairy cell leukemia.
Conclusions:
- Diminished CD19 expression is a frequent feature in B-cell lymphomas, particularly follicular lymphoma.
- Monitoring CD19 expression levels can be valuable for diagnosing B-cell lymphomas, especially in challenging cases like bone marrow aspirates.