Related Experiment Video
Updated: Aug 20, 2026
Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
Published on: March 25, 2016
[9-cis retinoic acid induces apoptosis in HL-60 cells]
1Department of Hematology, Beijing Tongren Hospital, Capital University of Medical Sciences, Beijing.
Objective:
To investigate the apoptosis of HL-60 cells induced by 9-cis retinoic acid (9-cis RA), and illustrate the possible molecular mechanism.
Methods:
Apoptosis was detected by morphological observation, DNA electrophoresis and flow cytometric cell cycle analysis. Bcl-2 expression was detected by flow cytometry.
Results:
9-cis RA initiated apoptosis of HL-60 cells after inducing them irreversibly commited to differentiation. Bcl-2 was downregulated during the differentiation and apoptosis process. 9-cis RA was more potent than all-trans retinoic acid (ATRA) did in inducing terminal differentiation associated apoptosis and in downregulation of Bcl-2 expression.
Conclusion:
9-cis RA can induce apoptosis in HL-60 cells. Downregulation of Bcl-2 expression appears to play an important role in the apoptosis of the differentiated leukemic cells.
