Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Involvement of gangliosides in glucosamine-induced proliferation decrease of retinal pericytes.

Elodie Masson1, Nicolas Wiernsperger, Michel Lagarde

  • 1Diabetic Microangiopathy Research Unit, Merck Santé-INSERM UMR 585, INSA-Lyon, Louis Pasteur Bldg, 69621 Villeurbanne Cedex, France.

Glycobiology
|December 31, 2004
PubMed
Summary

The hexosamine pathway (HP) activation, induced by glucosamine, reduces retinal pericyte proliferation. This effect is linked to increased gangliosides, particularly GM1 and GD3, suggesting their role in diabetic microvascular complications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Semi-mechanistic efficacy model for PARP + ATR inhibitors-application to rucaparib and talazoparib in combination with gartisertib in breast cancer PDXs.

British journal of cancer·2025
Same author

Ischemic Brain Injury: Involvement of Lipids in the Pathophysiology of Stroke and Therapeutic Strategies.

Antioxidants (Basel, Switzerland)·2024
Same author

Preclinical Pharmacokinetics and Translational Pharmacokinetic/Pharmacodynamic Modeling of M8891, a Potent and Reversible Inhibitor of Methionine Aminopeptidase 2.

Pharmaceutical research·2023
Same author

Correction: Impact of membrane lipid polyunsaturation on dopamine D2 receptor ligand binding and signaling.

Molecular psychiatry·2023
Same author

Model-based assessment of combination therapies - ranking of radiosensitizing agents in oncology.

BMC cancer·2023
Same author

Impact of dietary n-3 polyunsaturated fatty acid intake during the perinatal and post-weaning periods on the phospholipid and ganglioside composition of olfactory tissues.

Prostaglandins, leukotrienes, and essential fatty acids·2023

Area of Science:

  • Biochemistry
  • Cell Biology
  • Diabetology

Background:

  • Diabetic microvascular complications, like retinopathy, involve altered cell proliferation.
  • Retinal pericyte death is a hallmark of early diabetic retinopathy.
  • The role of gangliosides in diabetic microvascular alterations is understudied.

Purpose of the Study:

  • To investigate the impact of hexosamine pathway (HP) activation on retinal pericyte proliferation.
  • To determine the potential involvement of gangliosides in HP-mediated effects on pericytes.

Main Methods:

  • Mimicked HP activation using glucosamine treatment in retinal pericytes.
  • Analyzed ganglioside profiles following glucosamine treatment.
  • Assessed the effects of exogenous gangliosides and ganglioside biosynthesis inhibition (PPMP) on pericyte proliferation.

Related Experiment Videos

Main Results:

  • Glucosamine treatment decreased pericyte proliferation.
  • Glucosamine increased levels of GM1 and GD3 gangliosides.
  • Exogenous a-series and b-series gangliosides reduced pericyte proliferation, while precursors had no effect.
  • Inhibiting ganglioside synthesis partially reversed glucosamine's antiproliferative effect.

Conclusions:

  • Gangliosides, especially GM1 and GD3, are involved in the antiproliferative effect of glucosamine on retinal pericytes.
  • These findings suggest a role for gangliosides in the pathogenesis of diabetic microvascular complications.