Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inflammatory gene profiling in the developing mouse brain after hypoxia-ischemia.

Maj Hedtjärn1, Carina Mallard, Henrik Hagberg

  • 1Department of Physiology, Perinatal Center, Göteborg, University, Göteborg, Sweden. maj.hedtjarn@fysiologi.gu.se

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
|December 31, 2004
PubMed
Summary

Inflammation after brain hypoxia-ischemia in developing mice involves many genes, mostly upregulated. This study identifies novel inflammatory gene responses in the immature brain, crucial for understanding injury progression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond the gut: the multisite microbiota and its emerging role in brain health.

Gut microbes·2026
Same author

The role of persistent inflammation in failed recovery after perinatal brain injury: is resolution the cure?

Journal of neuroinflammation·2026
Same author

The Role of Serotonin in Brain Development: From Molecular Pathways to Neurodevelopmental Risk.

Cellular and molecular neurobiology·2026
Same author

Correction: Jonsdotter et al. MerTK and the Role of Phagoptosis in Neonatal Hypoxia-Ischemia. <i>Cells</i> 2025, <i>14</i>, 1862.

Cells·2026
Same author

Folate Receptor Alpha (FRα) and the Developing Brain: From Molecular Function to Neurodevelopmental Outcomes.

Molecular neurobiology·2026
Same author

Three-dimensional approaches to measuring primary cilia in hippocampal neurons: A comparative analysis.

Journal of neuropathology and experimental neurology·2026

Area of Science:

  • Neuroscience
  • Immunology
  • Developmental Biology

Background:

  • Brain ischemia induces inflammation, contributing to secondary injury progression.
  • Inflammatory responses and their effects differ between immature and adult brains.
  • Understanding inflammation in the developing brain is key to managing hypoxia-ischemia injury.

Purpose of the Study:

  • To characterize global inflammatory gene expression in the developing brain after hypoxia-ischemia.
  • To identify novel inflammatory pathways involved in immature brain injury.
  • To differentiate inflammatory responses in the immature brain compared to adults.

Main Methods:

  • Hypoxia-ischemia was induced in 9-day-old mice.
  • Oligonucleotide arrays analyzed inflammatory gene expression in brain regions (cortex, hippocampus, thalamus, striatum).

Related Experiment Videos

  • Gene expression was assessed at 2, 8, 24, and 72 hours post-injury.

    • Main Results:

    • 148 inflammatory genes were differentially expressed after hypoxia-ischemia.
    • Over 97% of these genes were upregulated, with 93% being novel findings in immature brain injury.
    • Immune cells (microglia, T/B cells, NK cells, etc.) and novel pathways (cytokines, complement, interferon, NF-kappaB) were implicated.

    Conclusions:

    • The immature brain exhibits a unique and extensive inflammatory gene expression profile post-hypoxia-ischemia.
    • Novel inflammatory genes and pathways are activated, potentially contributing to secondary injury.
    • These findings are significant for understanding and potentially treating neonatal hypoxia-ischemia.