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Updated: Aug 20, 2026
Intra-Peritoneal Transplantation for Generating Acute Myeloid Leukemia in Mice
Published on: January 6, 2023
[The anti-leukemia effects of IL-2 and IL-3 genes co-transfected leukemia vaccine]
1Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433.
Objective:
To investigate the anti-leukemia activity of IL-2 and IL-3 genes co-transfected leukemia vaccine.
Methods:
The vaccine was prepared with FBL-3 erythroleukemia cells which were in vitro transfected with IL-2 and/or IL-3 genes by recombinant adenovirus and then inactivated by 60Co irradiation. The vaccine was injected peritoneally into FBL-3 leukemia mice, and the tumor growth, survival time of the mice, cytotoxicities of the peritoneal macrophages, splenic NK cells and CTL cells were observed.
Results:
A fter treatment with the vaccine, the leukemic cell tumor in the mice showed significant growth inhibition and the survival time of the mice were prolonged when treated with IL-2 transfected vaccine, the cytotoxicities of splenic NK and CTL were increased significantly, while in the case of IL-3 transfected vaccine, the number and cytotoxicity of peritoneal macrophages were increased. The antileukemia effect was augmented when treated with IL-2 and IL-3 genes co-transfected vaccine. The antileukemia activity of the vaccine was also augmented by low dose cyclophosphamide administration.
Conclusion:
Leukemia vaccine gained more antitumor effect via induction of antitumor immunity.
