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[Study on the coagulant activity of human blood monocytes]
1Institute of Hematology, Beijing People's Hospital, Beijing Medical University, Beijing 100044.
Objective:
To investigate the coagulant and fibrinolytic activities of human blood monocytes.
Methods:
Cultured monocytes (2. 8 x 10(6) cells) were refrigerated at -20 degrees C and then thawed and centrifuged. Blood coagulant factors activities in the upper and lower layer of the supernatant were assayed and compared with that in the supernatant of white blood cells (containing 2. 8 x 10(6) cells).
Results:
There were factors II , VII, IX, X, V, and tissue factor activities in the supernatant, X III A : Ag was 2.1%, X III S : Ag : 2.8%, while t-PA : A and PAI : A were undetectable. In the lower layer there was no specific expression of blood coagulant factors.
Conclusion:
There were coagulant activities in the supernatant containing the membrane and cytoplasma of monocytes.
