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[Studies on the engraftment of human mixed cord blood in severe combined immunodeficient mice]
Objective:
To evaluate the engrafting characteristics of human mixed cord bloods.
Methods:
Single or double human cord bloods were transplanted into sublethally irradiated severe combined immunodeficient (SCID) mice. Engrafting rate, hematopoiesis reconstitution and immunologic functions between the two groups were compared.
Results:
The survival rates of both transplanted groups were equivalent (P > 0.05) while the total volume of transfused cord bloods had no statistical differences. But the engrafting rate of mixed cord blood transplant (MCBT) group was lower than that of single cord blood transplant (SCBT) group. The lymphocyte transformation rate of MCBT group was also lower than that of SCBT group. In MCBT group, only one of the two mixed cord bloods was engrafted in SCID mice as shown by HLA-DPB gene detection.
Conclusion:
Two HLA-mismatched human cord bloods transplanted in SCID mice could reconstitute hematopoietic and immunologic functions, but only one cord blood with the higher proliferation potential was engrafted.
