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[The implication of circulating CD34+ Thy-1(+) cells measurement during autologous peripheral blood stem cell
1Department of Hematology, The Third Clinical School of Medicine, Beijing Medical University, Beijing 100083.
Objective:
To estimate the amount of peripheral blood stem cells(PBSC) right during mobilization and determine the optimal time for PBSC collection.
Methods:
A serial measurement of circulating CD34+ Thy-1(+) cells were carried out during PBSC mobilization by chemotherapy and granulocyte colony stimulating factor(G-CSF) using dual-color direct immunofluorescence flow cytometry. Colony-culture in vitro was performed at the same time to evaluate the clonogenic capacity of the peripheral blood progenitor cells (PBPCs).
Results:
Circulating CD34+ Thy-1(+) cells, CD34+ cells and colony-forming cells increased by 48.6-, 50- and 53.1- fold, respectively, after mobilization. The peak levels were reached at 12 approximately 14 days after chemotherapy. The proportion of CD34+ cells and the candidate stem cells, CD34+ Thy-1(+) subset, in mononuclear cells (MNCs) exceeded baseline by 10.5- and 13.8- fold, respectively. The percentage of CD34+ cells coexpressing Thy-1 was highest in the earlist phase of mobilization.
Conclusion:
PBSCs, especially primitive progenitor cells, increased significantly during mobilization with chemotherapy and G-CSF. Flow cytometric assay of circulating CD34 Thy-1(+) cells promptly provides a more reliable indicator for clinical collection of PBSC.
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