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[The killing effect of IL-2 and IFN-alpha activated bone marrow on K562 leukemic cell]
1Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020.
Objective:
To evaluate the killing effect of IL-2 and IFN-alpha activated bone marrow cells on K562 cells.
Methods:
Semi-solid colony assay was used.
Results:
Bone marrow from leukemia patients in remission was activated in vitro with IL-2 for 3 days. The activated bone marrow (ABM) displayed killing effects of 0.31 approximately 2.30 logs on K562 cells, this killing effects was further increased to 0. 30 approximately > 3.15 logs when IFN-alpha added with IL-2 to the marrow for activation. IL-2 alone or in combination with IFN-alpha showed no inhibition of CFU-GM and K562 cells. Compared with IL-2 or IFN-alpha alone, the combination of the two cytokines could more effectively maintain the killing effect of ABM on leukemic cells.
Conclusion:
IFN-alpha can augment the purging effect of IL-2 ABM and combination of the two cytokines can effectively maintain the cytotoxicity of ABM.
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