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Updated: Aug 20, 2026
A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders
Published on: June 8, 2014
[Liposome-mediated gene transfer into bone marrow cells and its in vitro expansion]
Objective:
To explore the efficiency of liposome-mediated gene transfer into bone marrow cells and the stability of its expression.
Methods:
Two marker genes (Neo and Lac Z) mediated by liposome were co-transferred into bone marrow cells. Following G418 screening,the positive cells were enriched and expanded in vitro.
Results:
The liposome-mediated gene transfer rate in bone marrow cells was 13.33 +/- 2.68%, and after G418 screening, it was 46.06 +/- 3.47%. The blue colouring rates with X-gal staining of these cells were not significantly different before and after expansion.
Conclusion:
Liposome-mediated gene transfer in bone marrow cells is highly efficient and the expression of the transferred gene is stable.
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