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Updated: Aug 20, 2026

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
Published on: February 25, 2017
DNA-directed in vitro synthesis of elongation factor Tu
1Department of Biochemistry, Roche Institute of Molecular Biology, Nutley, New Jersey 07110, USA.
Abstract:
The in vitro synthesis of EF-Tu was studied using the transducing phage lambdarifd18 DNA as template. The EF-Tu synthesized was identified by its immunological properties, gel analysis, and its ability to interact with GDP and EF-Ts. Two other unidentified polypeptides were also precipitated from the incubation mixture by EF-Tu antiserum. The synthesis of total EF-Tu immunoprecipitable material was depressed about one-half by guanosine-5'-diphosphate-3'-diphosphate which was shown to inhibit the transcription process.
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