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Updated: Aug 20, 2026
Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
Particulate DNA polymerase from the cytoplasm of Xenopus laevis oocytes
M I Baldi1, P Bazzicalupo, G P Tocchini-Valentini
1Laboratory of Cell Biology, C. N. R., Via Romagnosi 18A, 00196 Rome, Italy.
Abstract:
A DNA polymerase has been partially purified and characterized from Xenopus laevis stage 6 oocytes. The enzyme is present only in the cytoplasm and has been shown to be able to copy Poly(A) x oligo(dT), to be sensitive to N-ethylmaleimide, and to sediment faster than 4 S in high salt glycerol gradient. The enzyme can be extracted from particulate material which has a density in sucrose gradient ranging from 1.200 to 1.225 g/cc. This particulate material is identified by its ability to use Poly(A) x oligo(dT) as template in an exogenous DNA polymerase reaction and by its endogenous DNA synthesizing capacity.
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