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Updated: Aug 20, 2026
Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
Published on: October 23, 2018
Serum albumin biosynthesis and secretion by resting and lectin stimulated human lymphocytes
Y Goussault1, A Sharif, R Bourrillon
1Centre de Recherches sur les Protéines, Faculté de Médecine Lariboisière-Saint Louis, Universitê Paris VII, 45 Rue des Saints Pères, Paris, France.
Normal human peripheral lymphocytes, cultured in serum-deprived medium, synthetized and released serum albumin and some glycoproteins into the culture supernatant. With the use of [3H]leucine, it was shown that this biosynthetic activity was increased about 2-3 times when the mitogenic lectin from Robinia pseudo acacia was added to the lymphocyte culture medium.
Normal human peripheral lymphocytes, cultured in serum-deprived medium, synthetized and released serum albumin and some glycoproteins into the culture supernatant. With the use of [3H]leucine, it was shown that this biosynthetic activity was increased about 2-3 times when the mitogenic lectin from Robinia pseudo acacia was added to the lymphocyte culture medium.
