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Updated: Aug 20, 2026
Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example
Published on: December 3, 2014
[Expression of iron responsive element binding protein mRNA and analysis of aconitase activity in iron deficiency
1Department of Hematology, Second Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041.
Objective:
To explore the regulatory mechanism of iron responsive element binding protein (IRE-BP) in iron metabolism.
Methods:
The expression level of IRE-BP mRNA and the aconitase activity were studied in iron deficiency anemia (IDA) rat model.
Results:
No distinct change of IRE-BP mRNA expression was observed in iron deficiency rats. The aconitase activity of liver cytoplasmic protein was much lower in iron deficiency rats than that in controls and treatment of liver cytoplasmic protein with FeSO4 resulted in a strong stimulation of the enzymatic activity.
Conclusion:
Both posttranslational and posttranscriptional regulation of IRE-BP may take part in iron metabolism balance.