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Complete cDNA sequence of bovine alpha 1-antitrypsin
D Sinha1, M R Bakhshi, E P Kirby
1Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA 19140.
Biochimica Et Biophysica Acta
|March 24, 1992
Summary
Researchers isolated and sequenced the bovine alpha 1-antitrypsin (AAT) gene. The bovine AAT shows high sequence homology with sheep and preserves critical structural elements found in other species.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Alpha 1-antitrypsin (AAT) is a crucial serine protease inhibitor.
- Understanding AAT structure and function across species aids in evolutionary and functional studies.
Purpose of the Study:
- To isolate and characterize the complete coding sequence of bovine alpha 1-antitrypsin (AAT).
- To compare the bovine AAT sequence with homologous sequences from other mammals.
Main Methods:
- Isolation of a cDNA clone encoding bovine AAT from a bovine liver cDNA library using a human AAT cDNA probe.
- DNA sequencing using the dideoxynucleotide chain termination method.
- Bioinformatic analysis and comparison of translated amino acid sequences.
Main Results:
- The complete coding sequence for bovine AAT was successfully obtained.
- Bovine AAT shares 94% sequence homology with sheep AAT.
- Bovine and sheep AAT possess four cysteine residues, unlike other mammals studied.
Conclusions:
- The bovine AAT gene sequence is highly conserved, particularly with sheep.
- Key structural determinants are preserved across mammalian AAT orthologs.
- The presence of four cysteine residues in bovine and sheep AAT may indicate specific functional or structural roles.