[Polymorphism analysis of the GOLA-DRB3 gene digested with HaeIII in Mongolian goat and Kazakh goat]
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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Dong-Xiao Sun1, Yuan Zhang, Ning Li
1College of Animal Science And Technology, China Agricultural University, Beijing 100094, China. sundx@cau.edu.cn
Abstract:
The exon2 of GOLA-DRB3 gene was amplified and a uniform fragment of 285 bp was obtained in Mongolian Goat and Kazakh Goat. The 285bp PCR product was digested with restriction endomuclease HaeIII and genetic polymorphism was investigated by PCR-RFLP. Seventeen kinds of genotypes were found in two populations,which were controlled by seven alleles. There are significant differences in some genotypic frequencies and gene frequencies between the two populations (P<0.10, P<0.05, P<0.01); The results of chi(2) test showed that genotypes of GOLA-DRB3 gene in two populations did not fit with Hardy-Weinberg equilibrium (P<0.01).
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