Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Odorant-specific requirements for arrestin function in Drosophila olfaction.

C Elaine Merrill1, Tracy M Sherertz, William B Walker

  • 1Department of Biological Sciences, Vanderbilt University, 465 21st Avenue South, Nashville, Tennessee 37232, USA.

Journal of Neurobiology
|January 1, 2005
PubMed
Summary

Arrestins are crucial for fruit fly olfaction, regulating odor sensitivity and processing. Flies lacking arrestins show reduced responses to various odors, impacting their ability to navigate chemical environments.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

eDNA analysis of yard waste samples reveals taxonomical diversity, sequence database limitations, and consistencies across sequencing platforms.

Journal of insect science (Online)·2026
Same author

Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.

Biological research·2025
Same author

Functional Characterization of a Female-Biased Chemoreceptor of the Codling Moth (Cydia pomonella) Responding to Aldehydes and Other Volatile Compounds.

Journal of chemical ecology·2025
Same author

Gene expression profiling of Cacopsylla pyricola (Hemiptera: Psyllidae) infected with Ca. Phytoplasma pyri (Acholeplasmatales: Acholeplasmataceae) reveals candidate effectors and mechanisms of infection.

Environmental entomology·2024
Same author

Using eDNA to play whack-a-mole with invasive species in green yard waste.

Journal of economic entomology·2024
Same author

Age and Task Modulate Olfactory Sensitivity in the Florida Carpenter Ant <i>Camponotus floridanus</i>.

bioRxiv : the preprint server for biology·2023

Area of Science:

  • Neuroscience
  • Sensory Biology
  • Genetics

Background:

  • Olfactory sensitivity requires precise modulation to detect chemical gradients and discriminate odors.
  • Arrestins are known to desensitize odorant receptors by inhibiting downstream signaling, but in vivo data is limited.
  • Investigating arrestins' role in olfactory desensitization is essential for understanding peripheral odor coding.

Purpose of the Study:

  • To investigate the in vivo role of arrestins in regulating olfactory signaling pathways using Drosophila melanogaster.
  • To determine the physiological and behavioral consequences of arrestin deficiency on olfactory function.
  • To elucidate the stimulus-specific requirements of arrestins in odor processing.

Main Methods:

  • Physiological analysis of peripheral olfactory sensitivity in flies deficient for arrestin1 (arr1) and arrestin2 (arr2).

Related Experiment Videos

  • Assessment of olfactory adaptation properties under prolonged odor exposure.
  • Behavioral experiments measuring olfactory-based orientation towards attractive odor sources.
  • Genetic rescue experiments involving transgenic expression of wild-type arr1.

    • Main Results:

    • Flies deficient in arr1, arr2, or both exhibited decreased olfactory responsiveness to diverse odorants in an odorant- and dose-dependent manner.
    • Mutant flies displayed altered adaptive properties following prolonged odor exposure.
    • arr1 mutants showed impaired olfactory-based orientation towards attractive odor sources.
    • Phenotypic rescue was achieved through transgenic expression of wild-type arr1 in arr1(1) mutants.

    Conclusions:

    • Arrestins are essential for wild-type olfactory function in a stimulus-specific manner.
    • Arrestin activity is required for proper odor processing across different olfactory pathways.
    • These findings reveal a new layer of complexity in peripheral odor coding mechanisms impacting olfactory behavior.