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Inhibition of soybean urease by triketone oximes
E I Tarun1, D B Rubinov, D I Metelitza
1Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk 220141, Belarus.
Abstract:
Competitive inhibition of soybean urease by 15 triketone oximes has been studied at 36 degrees C in aqueous solution (pH 4.95). The studied oximes are supposed chelators for the nickel atom in the urease metallocenter. The inhibition constants of urea hydrolysis (K(i)) varied in the range 2.7-248 microM depending on the oxime structure. Analysis of this dependency demonstrates that the optimal inhibitor is the one containing carbonyl group in position 1 of the cycle, the ethoxyimino group and alkyl residue in the substituent in position 2, as well as the methoxycarbonyl group in position 4 of the cycle.
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