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Updated: Jul 13, 2026

Development of Metarhizium anisopliae as a Mycoinsecticide: From Isolation to Field Performance
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Design and evaluation of a grapevine pruner for biofungicide application.

M A Ho1, L M Squire, N C Sabeh

  • 1Department of Biological and Agricultural Engineering, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA.

Bioresource Technology
|January 4, 2005
PubMed
Summary

A modified pruning shear effectively applies Fusarium lateritium to prevent Eutypa lata infection in grapevines. This biological control method offers a promising alternative for grapevine disease management.

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Area of Science:

  • Plant Pathology
  • Agricultural Engineering
  • Biological Control

Background:

  • Eutypella lata poses a significant threat to grapevines, with limited control options.
  • Fusarium lateritium is a biological control agent effective against Eutypella lata when applied to pruning wounds.

Purpose of the Study:

  • To develop and evaluate a novel application method for Fusarium lateritium using a modified pruning shear.
  • To assess the efficacy of this method in preventing Eutypella lata infection in grapevines.

Main Methods:

  • A commercial pruning shear was modified to dispense an invert emulsion formulation of Fusarium lateritium.
  • The modified pruner's wound coverage capability was tested.
  • The biological efficacy of the formulated agent applied via the pruner was evaluated on pruned grapevine canes.

Main Results:

  • The modified pruning shear successfully applied the Fusarium lateritium emulsion to grapevine pruning wounds.
  • Addition of grooves to the pruning blade significantly enhanced wound coverage.
  • Application of formulated Fusarium lateritium using the modified pruner demonstrated comparable efficacy to direct pipetting.

Conclusions:

  • A modified pruning shear provides an effective means for simultaneous application of Fusarium lateritium during grapevine pruning.
  • This integrated approach offers a practical and efficient strategy for biological control of Eutypella lata in vineyards.