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Published on: April 11, 2016
Characterisation of iota-carrageenans oligosaccharides with high-performance liquid chromatography coupled with
A Antonopoulos1, P Favetta, M Lafosse
1Institut de Chimie Organique et Analytique (ICOA), UMR CNRS 6005, Université d'Orléans, BP 6759, 45067 Orléans Cedex 2, France.
Abstract:
Enzymatically digested oligo-iota-carrageenans were separated with liquid chromatography, coupled to evaporative light scattering detection. As expected, compared to oligo-kappa-carrageenans, the additional sulphate group in the neocarrabiose unit of iota-carrageenans significantly modified the separation mechanisms on ion-exchange chromatography, porous graphitic carbon and ion-pair chromatography. The oligomers were then isolated and characterised off-line with electrospray ionisation mass spectrometry in positive-ion mode. The tetrasaccharide, hexasaccharide and octasaccharide that were identified were associated with protonated heptylamine molecules whose number depended on the number of sulphate groups.
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