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Updated: Aug 20, 2026
Generation of Monoclonal Antibodies Against Natural Products
Published on: April 6, 2019
[Preparation and characterization of monoclonal antibody against ARL-1 protein]
Jun Shan1, Jian-Qiong Zhang, Da Ma
1Genetics Research Center, Medical College of Southeast University , Nanjing 210009, China.
Aim:
To prepare monoclonal antibodies against aldose reductase-like(ARL-1)protein.
Methods:
Purified ARL-1 protein (ARL-GST) was used to immunize BALB/c mice, and mAbs were prepared by hybridoma technique. The mAbs against ARL-1 were detected by ELISA, Western blot and immunohistochemical staining respectively.
Results:
One clone of hybridoma secreting specific mAb against ARL-1 was obtained. The Ig subclass of mAb was of IgG2b and the titre of the antibody in ascites was 1:4.096 x 10(5). Highexpression of ARL-1 protein in liver cancer was detected.
Conclusion:
The specific mAb against ARL-1 lays the foundation for investigation of functions of ARL-1 protein and relationship between ARL-1 protein and liver cancer.
