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Updated: Aug 20, 2026

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
Published on: February 1, 2017
[Study on inhibitory effect of CpG-ODN on HBV replication in vitro]
Ning Li1, Xue-Gong Fan, Zhao-Hui Chen
1Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China. nxli@hotmail.com
Aim:
To study the inhibitory effect of synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG dinucleotides (CpG-ODN) on hepatitis B virus(HBV) replication in vitro.
Methods:
CpG-ODN was used to stimulate peripheral blood mononuclear cells(PBMCs). The IFN-alpha and IFN-gamma levels in the culture supernatant of stimulated PBMCs were measured by ELISA. The culture supernatant was added to HBV-transfected HepG2.2.15 cells, and after 2, 4, 6 and 8 days, HBsAg, HBeAg and HBV DNA levels in culture supernatant of 2.2.15 cells were detected by ELISA and quantitative PCR respectively.
Results:
The CpG-ODN alone could not inhibit HBV replication, whereas the culture supernatant of PMBCs activated with CpG-ODN could remarkably inhibit the production of HBsAg, HBeAg and HBV DNA by 2.2.15 cells, reaching the maximal inhibitory rates of 90.8%, 31.3% and 32.2%, respectively on the 8th day.
Conclusion:
The study indicates that, as a novel immunoregulator, CpG-ODN can inhibit indirectly HBV replication in vitro.
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