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Updated: Jan 31, 2026

Intraoperative Visualization of Subretinal Injection and Retinal Detachment in Rats
Published on: March 7, 2025
Retinal blood flow analysis using intraoperative video fluorescein angiography combined with optical fiber-free
Naoichi Horio1, Masayuki Horiguchi
1Department of Ophthalmology, Fujita Health University School of Medicine, Aichi, Japan. naoichi@fujita-hu.ac.jp
Purpose:
To report a new method to perform intraoperative video fluorescein angiography (VFA) using an optical fiber-free intravitreal surgery system (OFFISS) and retinal blood flow analysis during surgery.
Design:
Observational case series.
Methods:
Four eyes of four patients with macular edema as a result of branch retinal vein occlusion underwent vitrectomy and intraoperative VFA with a bolus of 0.8 ml fluorescein dye. The segmental blood flow was calculated using dye dilution technique. Intraocular pressure was maintained at 15 mm Hg.
Results:
The VFA was clearly recorded with stable excitation light and very little eye movement under retrobulbar anesthesia. The segmental blood flow of the affected vessels (6.1 +/- 1.2 pixel(2)/s) was significantly lower than that of the healthy vessels (17.9 +/- 7.9 pixel(2)/s) (P < .05).
Conclusion:
The combination of VFA and OFFISS can facilitate intraoperative VFA, and may be useful for evaluating retinal blood flow during surgery.
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