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Updated: Aug 20, 2026

09:50
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
Measurement of isoflavones using liquid chromatography with multi-channel coulometric electrochemical detection
Kazuyuki Ouchi1, Paul Gamache, Ian Acworth
1Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-0054, Japan.
Biofactors (Oxford, England)
|January 5, 2005
Abstract:
We evaluated the use of HPLC multi-channel coulometric electrochemical detection (HPLC-ECD) for the routine measurement of phytochemicals by analyzing standards and urine samples. Compounds were separated chromatographically and electrochemically with high sensitivity (10 ng/ml of isoflavones in urine). HPLC-ECD will enable better investigation into the relationship between lifestyle and the effects of phytochemicals on humans.

