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Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...

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Updated: Jun 29, 2026

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
06:40

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

Published on: September 15, 2014

Dopaminergic neurons generated from monkey embryonic stem cells function in a Parkinson primate model.

Yasushi Takagi1, Jun Takahashi, Hidemoto Saiki

  • 1Department of Neurosurgery, Kyoto University Graduate School of Medicine, Kyoto, Japan.

The Journal of Clinical Investigation
|January 5, 2005
PubMed
Summary

Parkinson disease (PD) therapies show promise with embryonic stem (ES) cells. Researchers successfully generated dopamine (DA) neurons from monkey ES cells, which improved motor function in a primate model of PD.

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Last Updated: Jun 29, 2026

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
06:40

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

Published on: September 15, 2014

Primary Culture of Mouse Dopaminergic Neurons
11:58

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Published on: September 8, 2014

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Published on: July 11, 2019

Area of Science:

  • Neuroscience
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Parkinson disease (PD) involves the loss of midbrain dopaminergic (DA) neurons.
  • Embryonic stem (ES) cells are a promising source for cell-replacement therapy in PD.
  • Stromal cell-derived inducing activity (SDIA) has shown neuralizing potential.

Purpose of the Study:

  • To generate DA neurons from monkey ES cells for PD therapy.
  • To investigate the synergistic effects of FGF20 and FGF2 on DA neuron generation.
  • To assess the therapeutic efficacy of transplanted DA neurons in a primate PD model.

Main Methods:

  • Generation of neural progenitor neurospheres from monkey ES cells.
  • Utilizing stromal cell-derived inducing activity (SDIA) for neural induction.
  • Employing FGF20 and FGF2 to enhance DA neuron production.
  • Transplantation of ES cell-derived DA neurons into MPTP-treated monkeys.

Main Results:

  • Monkey ES cell-derived neurospheres produced substantial numbers of DA neurons.
  • FGF20 and FGF2 synergistically increased DA neuron yield.
  • Transplanted DA neurons integrated and functioned in the primate brain.
  • MPTP-induced neurological symptoms were attenuated following transplantation.

Conclusions:

  • Monkey ES cells can be differentiated into functional DA neurons.
  • FGF growth factors enhance DA neuron generation from ES cells.
  • ES cell-derived DA neuron transplantation shows therapeutic potential for Parkinson disease in primates.