Related Experiment Video
Updated: Aug 20, 2026
Laser Doppler Perfusion Imaging in the Mouse Hindlimb
Published on: April 18, 2021
He-Ne laser effects on blood microcirculation during wound healing: a method of in vivo study through laser Doppler
Silvia Cristina Núñez1, Gessé E C Nogueira, Martha S Ribeiro
1Centro de Lasers e aplicacoes-Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo, Brazil 05508-000. silnunez@aol.com
Background And Objectives:
Low-intensity laser therapy (LILT) is widely used for wound healing promotion and its mechanism of action may be due to an enhancement of blood supply. The aim of this study was to evaluate blood flow alterations in a wound healing model, using laser Doppler flowmetry (LDF) associated with a normalized perfusion parameter.
Study Design/Materials And Methods:
An injury was provoked in 15 rats and blood flow was measured periodically over a period of 21 days. Control groups were established to evaluate LDF and He-Ne laser effects on microcirculation. A 1 J/cm(2) dose was utilized, with 6 mW/cm(2) irradiance.
Results:
The results demonstrated flow alterations provoked by lesion, and inflammatory response (P < 0.05). There were no statistical differences between groups.
Conclusions:
The results did not show a significant sustained effect on microcirculation with this He-Ne dose.
