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[Study on ligustrazine in reversing multidrug resistance of HepG2/ADM cell in vitro]
Ying Mei1, Yu-jun Shi, Guo-qing Zuo
1The Department of Hepato-biliary Surgery, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
Objective:
To study the reverse effect of ligustrazine (TMP) on HepG2/ADM, a herd of hepatocellular carcinoma cell, multidrug resistance (MDR) and the influence of P-gp170 expression.
Method:
The reverse effect of ligustrazine on HepG2/ADM cell was observed, with the methods of cell culture, MTT's analyze, RT-PCR and Flow cytometric, etc.
Result:
Ligustrazine could make MDR of cell line of HepG2/ADM reduce the expression of P-gp170, enhance the density of adriamycin in cell and increase the adriamycin's cytotoxicity. With the Flow cytometric, the results of RT-PCR showed the transcriptional activity of the MDR1 decreased.
Conclusion:
Ligustrazine can reverse MDR of HCC cell line of HepG2/ADM and has prospect in clinical use.
