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Updated: Aug 20, 2026
Standardized Rat Coronary Ring Preparation and Real-Time Recording of Dynamic Tension Changes Along Vessel Diameter
Published on: June 16, 2022
[Endothelium-independent vasorelaxant effect of puerarin on rat thoracic aorta]
Kan Dong1, Qian-min Tao, Qiang Xia
1Department of Cardiovascular, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. dongkan76@sohu.com
Objective:
To investigate the vasorelaxant effect of puerarin in rat aortic rings and the mechanism.
Method:
The isolated thoracic aortic rings of male Sprague-Dawley rats were mounted on the organ bath and the contractile responses of the vessel were recorded.
Result:
Puerarin completely relaxed the contractions induced by phenylephrine in a concentration-dependent manner in endothelium-intact and endothelium-denuded rat aorta, but it had no effect on those preconstricted by a high concentration of potassium chloride (KCl, 60 mmol x L(-1)). The relaxant effect of puerarin was significantly inhibited by pretreatment of endothelium-denuded aorta with potassium channel antagonists tetraethylammonium, 4-aminopyridine but not glibenclamide.
Conclusion:
Puerarin induces an endothelium-independent relaxation in rat aortic rings. The mechanisms may involve the reduction in Ca2+ influx through the calcium channels operated by alpha-adrenergic receptor and the activation of the potassium channels (Kv and BKca, but not KATP).
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