[Analysis of genetic relationship of egg-type chickens using microsatellite markers]

Twenty microsatellite markers were used to analyse the genetic relationship among four package lines (A, B, C, D) of egg-type chickens introduced in 2001 an 2002. The genetic heterozygosity, polymorphism information content (PIC), effective allele number, and genetic distance among lines were calculated and dendrogram was constructed to evaluate the genetic variability within line and genetic relations among lines. In total, 65 alleles in 20 microsatellite markers were detected. Average allele number for microsatellite markers is 3.250, and average effective allele number for those markers is 2.395. The PIC of microsatellite markers averaged at 0.454, ranging from 0.102 to 0.729. The heterozygosity of microsatellite markers ranged from 0.108 to 0.765. The heterozygosity of A2001 (0.390) was lowest, and that of D2001 (0.452) highest. The genetic distances among A and B lines were 0.005-0.016, while those among C and D lines were 0.094-0.119. Genetic resemble coefficients among A and B lines were above 0.984, while those among C and D lines around 0.900. The results proved, on the molecular level, that A and B are identical or two lines but very closed in genetic background while C and D are two different lines.