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Published on: December 17, 2017
[Effect of luteinized granulosa cell conditioned medium on cortical granule of mouse oocytes matured in vitro]
Xiao-Hong Bai1, Ruo-Ran Mi, Tian-Fu Yue
1Department of Obstetrics and Gynecology, Tianjin Medical University General Hospital, Tianjin 300052, China.
Objective:
To study the effect of luteinized granulosa cell conditioned medium on cortical granule (CG) of the mouse oocytes matured in vitro.
Methods:
Oocytes in germinal vesicle (GV) stage of Kunming mice were randomly divided into 2 groups according to different in vitro maturation (IVM) culture media. The study group medium contained 50% granulosa cell condition medium, follicle stimulating hormone 75 U/L and estrodial 1 nmol/L. The control group medium contained follicle stimulating hormone 75 U/L and estrodial 1 nmol/L. Oocytes were cultured for 16 or 18 hours. CG was examined by fluorescein isothiocyanate labeled Lens culinaris agglutinin under a confocal scanning laser microscope.
Results:
After cultured for 16 hours, the nuclear maturation rates of control and study groups were 70.0% and 76.5%. After cultured for 18 hours, the maturation rates were 75.1% and 83.1%, respectively. There was no significant difference between the two groups. After cultured for 16 hours, there was no pronuclear formation in both groups. When culture was extended to 18 hours, fertilization occurred. After cultured for 16 hours, the rates of CGs forming a line under membrane were 10.0% and 50.0% in control and study groups respectively. When culture was extended to 18 hours, the rates rose to 57.1% and 91.6% accordingly. The rate of 18 h of each group was significantly higher than that of 16 h (both P < 0.001). The rate of study group of 18 h was significantly higher than that of control group (P < 0.05).
Conclusion:
Granulosa cell conditioned medium could improve the mouse oocytes maturation competence in vitro.
