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Updated: Jul 30, 2026
In Vitro Analysis of E3 Ubiquitin Ligase Function
Published on: May 14, 2021
Assay for ubiquitin ligase activity: high-throughput screen for inhibitors of HDM2
I V Davydov1, D Woods, Y J Safiran
1Meso-Scale Discovery, Meso-Scale Diagnostics, LLC, Gaithersburg, MD, USA.
Abstract:
An assay for the autoubiquitination activity of the E3 ligase HDM2 (Mdm2) was developed and adapted to a high-throughput format to identify inhibitors of this activity. The assay can also be used to measure the activity of other E3s and may be useful in finding both inhibitors and activators of a wide range of different ubiquitin ligases.
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