Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An improved method for checking HTS/uHTS liquid-handling systems.

Heidrun Rhode1, Margarete Schulze, Simon Renard

  • 1Institute of Biochemistry, Friedrich Schiller University, Medical Faculty, D-07740 Jena, Germany. Heidrun.Rhode@mti.uni.uni-jena.de

Journal of Biomolecular Screening
|January 7, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic and therapeutic laparoscopy in pediatric blunt abdominal trauma: a systematic review.

Updates in surgery·2026
Same author

Preservation vs. Resection? Pediatric and Non-Pediatric Management Patterns in Ovarian Torsion.

Pediatric reports·2026
Same author

Practitioner Review: Clinical insights from attachment theory and research for professionals working with young children and their families.

Journal of child psychology and psychiatry, and allied disciplines·2026
Same author

The hidden mechanism of vacuum bell therapy: Local fat hypertrophy drives cosmetic outcome in adolescents with pectus excavatum.

JPRAS open·2026
Same author

The Chemistry and Thermodynamics of Point Source CO<sub>2</sub> Capture by Liquid Chemical Absorption and Its Impact on Process Performance.

Chemical reviews·2025
Same author

Small lesion-high risk: diagnostic performance of artificial intelligence in paediatric fractures with medicolegal impact.

Pediatric radiology·2025
Same journal

Functional Comparison of Neuronal Cells Differentiated from Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells under Different Oxygen and Medium Conditions.

Journal of biomolecular screening·2017
Same journal

Development of an In Vitro Model to Screen CYP1B1-Targeted Anticancer Prodrugs.

Journal of biomolecular screening·2017
Same journal

Erratum.

Journal of biomolecular screening·2017
Same journal

Software, Database, and Information Services.

Journal of biomolecular screening·2017
Same journal

High-Throughput Platform for Identifying Molecular Factors Involved in Phenotypic Stabilization of Primary Human Hepatocytes In Vitro.

Journal of biomolecular screening·2016
Same journal

SLAS Europe High-Content Screening Conference in Dresden: A Glimpse of the Future?

Journal of biomolecular screening·2016
See all related articles

This study presents a new method for assessing liquid handling systems, achieving high precision for accurate volume delivery. The optical and gravimetric techniques ensure reliable performance for multichannel systems in various applications.

Area of Science:

  • * Laboratory automation and liquid handling technology.
  • * Analytical chemistry and assay development.

Background:

  • * Accurate and precise liquid handling is critical for reproducible experimental results in various scientific fields.
  • * Existing methods for assessing multichannel liquid handling systems may not fully reflect real-world application conditions.

Purpose of the Study:

  • * To develop and validate an efficient method for determining the precision and accuracy of multichannel liquid-handling systems.
  • * To evaluate the performance of the method under application-near conditions using different devices and microplate formats.

Main Methods:

  • * Gravimetrical determination of accuracy and optical determination of precision using diluted absorbing and fluorescent dye solutions in microplates.
  • * Multiwavelength measurements to improve optical signal precision (CV(S)).

Related Experiment Videos

  • * Performance evaluation using CyBi-Drop dispenser and CyBi-Well pipettor with various fluids and microplate formats (96, 384, 1536 wells).

    • Main Results:

    • * Mean delivery volume per well determined with precision better than 0.04% coefficient of variation (CV).
    • * Absorbance measurements offer better precision (0.3% CV) than fluorescence measurements (1.5% CV).
    • * Optimized methods achieved volume delivery precision better than 2% CV at 2 microL and about 1% CV for higher volumes.

    Conclusions:

    • * The presented method provides an efficient and reliable way to assess multichannel liquid-handling system performance.
    • * Absorbance-based optical measurements are recommended for superior precision determination.
    • * The method demonstrates high precision for volume delivery across different devices, plate formats, and volumes.