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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
08:51

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Comparison of emm typing and ribotyping with three restriction enzymes to characterize clinical isolates of

Stella Z Doktor1, Jill M Beyer, Robert K Flamm

  • 1Infectious Diseases Research, Abbott Laboratories, Abbott Park, IL 60064, USA. stella.z.doktor@abbott.com

Journal of Clinical Microbiology
|January 7, 2005
PubMed

Insights

This study characterized Streptococcus pyogenes isolates from pharyngitis patients using emm typing and riboprinting. The methods revealed distinct bacterial groups, aiding in understanding pathogen diversity and epidemiology.

Area of Science:

  • Microbiology
  • Molecular Epidemiology
  • Bacterial Typing

Background:

  • Streptococcus pyogenes is a significant cause of pharyngitis globally.
  • Accurate typing methods are crucial for tracking pathogen strains and understanding disease outbreaks.

Purpose of the Study:

  • To characterize a collection of Streptococcus pyogenes isolates from pharyngitis patients.
  • To evaluate the discriminatory power of emm typing and automated riboprinting for S. pyogenes.

Main Methods:

  • emm typing and automated riboprinting using EcoRI, PstI, and HindIII restriction enzymes were performed on 336 S. pyogenes isolates.
  • Multilocus sequence typing (MLST) was used for a subset of isolates to compare with other typing methods.

Main Results:

  • A total of 40 distinct ribogroups and 38 emm sequence types were identified.
  • Combining three restriction enzymes enhanced the discrimination of ribogroups.
  • Results from emm typing, riboprinting, and MLST showed strong correlations.

Conclusions:

  • Automated riboprinting, especially with multiple enzymes, is a valuable tool for characterizing S. pyogenes.
  • emm typing and riboprinting are consistent and complementary methods for molecular epidemiology of S. pyogenes.

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