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Evaluation of nested PCR in diagnosis of typhoid fever
Pradyot Prakash1, Om Prakash Mishra, Alok Kumar Singh
1Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India. pradyot_micro@yahoo.co.in
Journal of Clinical Microbiology
|January 7, 2005
Abstract:
In this study, nested PCR using H1-d primers, which is specific for Salmonella enterica serovar Typhi, was compared to blood culture and the single-tube Widal test. Results indicate that nested PCR can be used as a gold standard to determine the cutoff titer of the Widal test for diagnosis of typhoid fever.
