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Updated: Aug 20, 2026
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
Published on: October 16, 2018
Evidence for preferential genotyping of a minority human immunodeficiency virus population due to primer-template
Javier Martinez-Picado1, Kristina Morales-Lopetegi, Cristina Villena
1IrsiCaixa Foundation, Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain. javiermp@ns.hugtip.scs.es
Abstract:
Human immunodeficiency virus type 1 (HIV-1) genotyping assays have come to be widely used for monitoring antiretroviral drug resistance. We report a case in which primer-template mismatches during nested PCR-based amplification biased the composition of the original viral population in the sample, magnifying a distinct minority HIV-1 population. This observation might help to explain some unexpected HIV-1 genotypes.
