Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cloning a replication function from the streptomycete bacteriophage FP43.

M Howell1, R N Rao

  • 1Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN 46285-0424.

Gene
|April 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of the What Matters 2 Adults (WM2A) wellbeing measure for Aboriginal and Torres Strait Islander adults.

Social science & medicine (1982)·2024
Same author

What Matters to Aboriginal and Torres Strait Islander Youth (WM2Y): a study protocol to develop a national youth well-being measure.

BMJ open·2024
Same author

Feasibility and impact of a one-stop thyroid clinic in a low- and middle-income country.

South African journal of surgery. Suid-Afrikaanse tydskrif vir chirurgie·2022
Same author

An improved cleaning system to reduce microbial contamination of poultry transport crates in the United Kingdom.

Journal of applied microbiology·2020
Same author

Use of progression criteria to support monitoring and commissioning decision making of public health services: lessons from Better Start Bradford.

BMC public health·2019
Same author

Unusual association of leprosy with Lucio phenomenon with secondary antiphospholipid antibody syndrome and ischemic stroke.

Neurology India·2019

Researchers identified specific DNA fragments from bacteriophage FP43 that control plaque inhibition and replication in Streptomyces. These findings are crucial for understanding phage-host interactions and developing new genetic tools for Streptomyces.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Bacteriophage FP43 interactions with Streptomyces griseofuscus are not fully understood.
  • Genetic elements responsible for bacteriophage functions require detailed characterization.

Purpose of the Study:

  • To identify and characterize the DNA fragments of bacteriophage FP43 responsible for plaque inhibition (Pin) and replication (Rep) functions in Streptomyces.
  • To determine the stability and compatibility of plasmids carrying the FP43 replication function.

Main Methods:

  • Cloning of SphI DNA fragments from bacteriophage FP43 into Streptomyces.
  • Functional analysis of DNA fragments for plaque inhibition and replication.
  • Plasmid stability and copy number determination.

Related Experiment Videos

  • Compatibility studies with existing Streptomyces plasmids.

    • Main Results:

    • A 4.4-kb SphI DNA fragment from FP43 conferred plaque inhibition (Pin) in S. griseofuscus, with the Pin function localized to a 0.96-kb SacII fragment.
    • The same 4.4-kb SphI fragment also mediated autonomous replication (Rep) in Streptomyces, with the Rep function localized to a 1.2-kb SphI-FspI fragment.
    • Plasmids harboring the FP43 Rep function were unstable, existing at 20-50 copies per chromosome, and were compatible with SCP2* plasmids.

    Conclusions:

    • Specific DNA fragments of bacteriophage FP43 encode essential functions for plaque inhibition and replication in Streptomyces.
    • The FP43 replication system exhibits instability and specific compatibility characteristics, offering insights for genetic engineering in Streptomyces.