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Purification of restriction endonuclease EcoO128I produced by an enteropathogenic Escherichia coli O128Ly3
M Miyahara1, K Mise, F Kimizuka
1Division of Microbiology, National Institute of Hygienic Sciences, Tokyo, Japan.
Gene
|April 1, 1992
Abstract:
Restriction endonuclease EcoO128I, an isoschizomer of BstEII, was purified from a rough mutant of Escherichia coli O128Ly3. EcoO128I should be more convenient for recombinant DNA applications than BstEII, because of its improved cleavage activity at 37 degrees C.