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Related Experiment Videos

Ultraviolet light and lysosome activity.

K Aoki1, T Saito

  • 1Department of Dermatology, Osaka City University Medical School, Abeno-Ku, Osaka, 545 Japan.

The Journal of Dermatology
|April 1, 1976
PubMed
Summary
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Ultraviolet (UV) light exposure increases skin lysosome enzyme activity, specifically beta-glucuronidase and acid phosphatase. These UV-induced changes occur with less energy than required for visible skin redness (erythema).

Area of Science:

  • Dermatology
  • Biochemistry
  • Photobiology

Background:

  • Ultraviolet (UV) radiation from lamps can induce changes in skin.
  • Lysosomes are cellular organelles involved in enzymatic degradation.
  • Enzyme activity in skin can be affected by external stimuli like UV light.

Purpose of the Study:

  • To investigate the effects of specific UV light wavelengths on lysosome enzyme activity in guinea pig skin.
  • To compare the activity changes of acid phosphatase and beta-glucuronidase following UV irradiation.
  • To determine the energy threshold for UV-induced enzyme changes versus erythema.

Main Methods:

  • Guinea pig abdominal skin was irradiated using a Xenon short arc lamp.
  • Glass filters (UV-25, UV-27, UV-29, UV-31) were used to select specific UV wavelengths.

Related Experiment Videos

  • Activity levels of acid phosphatase and beta-glucuronidase were measured in the irradiated skin.

    • Main Results:

    • Both acid phosphatase and beta-glucuronidase activity increased in UV-irradiated skin.
    • Beta-glucuronidase showed a rapid and significant increase, while acid phosphatase exhibited a slower, milder rise.
    • Acid phosphatase activity was similarly affected by all tested UV filters, whereas beta-glucuronidase was less affected by the UV-31 filter.

    Conclusions:

    • Cutaneous changes induced by UV light are closely linked to alterations in lysosome enzyme activity.
    • The energy required to alter skin enzyme activity via UV light is substantially lower than that needed to cause erythema.
    • Different UV wavelengths may differentially impact specific lysosome enzymes.