Related Experiment Video

Updated: Aug 20, 2026

Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe
09:31

Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe

Published on: September 17, 2021

Potential oxidative stress in children with chronic constipation

Jun-Fu Zhou1, Jian-Guo Lou, Sheng-Li Zhou

  • 1Second Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang Province, China. jfzhou@zju.edu.cn

World Journal of Gastroenterology
|January 8, 2005
PubMed

Insights

Children with chronic constipation show signs of oxidative stress. Their levels of antioxidants like vitamin C and E decreased, while harmful lipoperoxides increased.

Area of Science:

  • Pediatric Gastroenterology
  • Biochemistry
  • Oxidative Stress Research

Background:

  • Chronic constipation is a common pediatric issue.
  • Oxidative stress is implicated in various chronic diseases.
  • The link between chronic constipation and oxidative stress in children requires further investigation.

Purpose of the Study:

  • To investigate potential oxidative stress in children suffering from chronic constipation.
  • To explore the underlying mechanisms of oxidative stress in this condition.

Main Methods:

  • A randomized controlled study involving 70 children with chronic constipation and 70 healthy controls.
  • Measurement of plasma levels of vitamin C and E.
  • Determination of erythrocyte superoxide dismutase and catalase activity, and lipoperoxide levels via spectrophotometry.

Main Results:

  • Children with chronic constipation exhibited significantly lower levels of vitamin C, vitamin E, superoxide dismutase, and catalase compared to healthy controls.
  • Elevated lipoperoxide levels were observed in children with chronic constipation.
  • A prolonged disease course correlated with decreased antioxidant levels and increased lipoperoxide levels.

Conclusions:

  • Chronic constipation in children is associated with significant oxidative stress.
  • The findings suggest a potential role for oxidative stress in the pathophysiology of chronic constipation in pediatric populations.
Abstract

More Related Videos

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro03:35

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro

Published on: June 28, 2024

Related Concept Videos

Chronic Obstructive Pulmonary Disease-II: Pathophysiology01:20

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
Chronic Bowel Disorders: Introduction01:17

Chronic Bowel Disorders: Introduction

Chronic bowel diseases are a group of long-term conditions affecting the digestive tract, characterized by inflammation and damage to the gut lining. These conditions primarily include irritable bowel syndrome and inflammatory bowel disease.
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
Assessment of the Rectum and Anus01:25

Assessment of the Rectum and Anus

Evaluating the rectum and anus plays a crucial role in conducting a thorough physical examination of the gastrointestinal system. Although it may be uncomfortable and often embarrassing for the patient, it holds immense diagnostic value, particularly in detecting gastrointestinal diseases and abnormalities. This guide will explain how to perform this assessment using inspection and palpation methods.
Rectal Inspection
Begin by inspecting the perianal and anal areas for color, texture, rashes,...