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Updated: Aug 20, 2026

Extraction and Analysis of Cortisol from Human and Monkey Hair
08:38

Extraction and Analysis of Cortisol from Human and Monkey Hair

Published on: January 24, 2014

Assay of cortisol in serum by competitive protein binding without solvent extraction

R Morris1, G Holder

  • 1Department of Clinical Endocrinology, The Women's Hospital, Showell Green Lane, Sparkhill, Birmingham B11 4HL.

Annals of Clinical Biochemistry
|November 1, 1975
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.