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Updated: Aug 20, 2026

Isolation of Murine Lymph Node Stromal Cells
Published on: August 19, 2014
Nonlymphoid peritoneal cells suppress the T cell response to Mls
Laura Rosini1, Robin Matlack, Justin Taylor
1Department of Biology, Rider University, Lawrenceville, NJ 08648-3099, USA.
Abstract:
Comparative analyses of the ability of lymphoid tissue to present the minor lymphocyte stimulatory (Mls) superantigen Mls-1a in vitro revealed that all tissues containing mature B cells, except peritoneal cavity (PerC) cells, induced Mls-1a-specific T cell activation. Irradiation and mitomycin C treatment, addition of IL-2 and IL-12, and neutralization of IL-10 and TGF-beta did not restore Mls-1a antigen presentation by PerC cells. Co-culture studies revealed that PerC cells actively suppress the T cell response to Mls-1a. PerC cells from severe-combined immune-defective (SCID) mice also suppressed this response indicating that nonlymphoid cells mediate this effect. These results suggest that in addition to antigen processing and presentation, resident peritoneal cavity cells may temper lymphocyte activation.
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