Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Techniques of image analysis for quantitative immunohistochemistry.

E Kaczmarek1, A Górna, P Majewski

  • 1Laboratory of Morphometry and Medical Image Processing, University of Medical Sciences, Poznań, Poland. elka@amp.edu.pl

Roczniki Akademii Medycznej W Bialymstoku (1995)
|January 11, 2005
PubMed
Summary

Digital image analysis offers a new way to measure immunohistochemical markers. This study compares a spatial visualization technique against color sampling and grayscale thresholding for improved accuracy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A prototype differential atom interferometer for fundamental physics.

Nature·2026
Same author

Human skeletal muscle-derived stem/progenitor cells modified with connexin-43 prevent arrhythmia in rat post-infarction hearts and influence gene expression in the myocardium.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2020
Same author

Long-term survival in HER2-positive metastatic breast cancer treated with first-line trastuzumab: results from the french real-life curie database.

Breast cancer research and treatment·2019
Same author

First Results from the DEAP-3600 Dark Matter Search with Argon at SNOLAB.

Physical review letters·2018
Same author

The Gene-Diet Associations in Postmenopausal Women with Newly Diagnosed Dyslipidemia.

The journal of nutrition, health & aging·2017
Same author

Analysis of immunohistochemical expression of proinflammatory cytokines (IL-1α, IL-6, and TNF-α) in gallbladder mucosa: comparative study in acute and chronic calculous cholecystitis.

Folia morphologica·2015

Area of Science:

  • Biomedical Imaging
  • Histopathology
  • Quantitative Analysis

Background:

  • Immunohistochemistry (IHC) is crucial for biomarker quantification in research and diagnostics.
  • Accurate measurement of IHC marker amount and strength is essential for reliable results.
  • Existing methods for IHC analysis can be subjective or lack precision.

Purpose of the Study:

  • To assess the utility of digital image analysis for quantifying IHC markers.
  • To compare a novel spatial visualization technique with established methods.
  • To demonstrate applications in apoptosis and proliferation marker analysis.

Main Methods:

  • Digital image analysis using spatial visualization technique.
  • Comparison with color sampling and grayscale thresholding methods.

Related Experiment Videos

  • Application to immunohistochemical staining for apoptosis and proliferation markers.

    • Main Results:

    • The spatial visualization technique demonstrated effectiveness in measuring IHC marker characteristics.
    • Quantitative comparison with color sampling and grayscale thresholding provided insights into method performance.
    • Successful application examples for apoptosis and proliferation markers were presented.

    Conclusions:

    • Digital image analysis, particularly spatial visualization, offers a valuable tool for objective IHC marker quantification.
    • This technique enhances the reliability and precision of biomarker assessment.
    • The presented methods have broad applicability in various research areas utilizing IHC.