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A blue dye for substrate tagging in the two-color screening of combinatorial libraries

Vicente del Amo1, Adam P McGlone, Clive Foster

  • 1School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK.

Journal of Combinatorial Chemistry
|January 11, 2005
PubMed
Abstract

No abstract available in PubMed .

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...