Related Experiment Video
Updated: Aug 20, 2026

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
Published on: April 25, 2025
An investigation into the excitation migration in polyfluorene solutions via temperature dependent fluorescence
Helen L Vaughan1, Fernando M B Dias, Andrew P Monkman
1OEM Research Group, Department of Physics, University of Durham, Durham DH1 3LE, England. h.l.vaughan@dur.ac.uk
Abstract:
Fluorescence anisotropy of dilute polyfluorene polymer solutions has been used to show that two processes, exciton migration and conformational relaxation (twisting of part of the chain), occur within polyfluorene polymers of 20 or more repeat units. The former process is dominant and temperature independent for high excitation energy but, as the chain length is decreased, exciton migration is eliminated and the conformational relaxation becomes the only mechanism by which excited state energy relaxation to the emission site can occur. When the polymers are excited in the absorption band tails, again no migration is observed but conformational relaxation is still present.
Related Concept Videos
Variables Affecting Phosphorescence and Fluorescence
Fluorescence and Phosphorescence: Instrumentation

