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Updated: Aug 20, 2026

Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
Published on: August 11, 2018
Are oblique orientated alpha-helices used by antimicrobial peptides for membrane invasion?
Sarah R Dennison1, Frederick Harris, David A Phoenix
1Department of Forensic and Investigative Science, University of Central Lancashire, Preston, UK.
Abstract:
Oblique orientated alpha-helices are highly specialised protein structural elements that penetrate membranes at a shallow angle and are used to promote membrane destabilisation by a number of protein classes. Here, the use of extended hydrophobic moment methodology shows that the amphibian extrudates, aurein 1.2 and citropin 1.1, may use oblique orientated alpha-helices in their antimicrobial action and that such use may be shared by other antimicrobial peptides. This appears to be the first systematic analysis of these peptides for the possession of oblique orientated alpha-helical structure.
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