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Published on: August 23, 2019
Polysaccharides isolated from Phellinus gilvus inhibit melanoma growth in mice
Jae-Sung Bae1, Kwang-Ho Jang, Hyunee Yim
1Department of Surgery, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, South Korea.
Abstract:
There is no information about the effect of polysaccharides from fungus, Phellinus gilvus (PG) on melanoma. The effect of PG on the proliferation and apoptosis of the B16F10 melanoma cell line was determined by a sulforhodamine B (SRB) and a sandwich enzyme-linked immunosorbent assay. The in vivo effect of PG on B16F10 melanoma cells allografted in athymic nude mice was investigated. PG decreased cell proliferation and increased cell apoptosis in a dose dependent manner in vitro. Also, PG significantly inhibits melanoma growth in mice. The PG anti-tumor effect in vivo was associated with a significant increase in the melanoma apoptosis rate. These findings support PG as a therapeutic agent against melanoma.
Insights
Polysaccharides from Phellinus gilvus (PG) show therapeutic potential against melanoma. This fungus extract inhibits melanoma cell growth and induces apoptosis in vitro and in vivo.
Area of Science:
- Mycology
- Immunology
- Oncology
Background:
- Melanoma is a significant form of skin cancer.
- Limited research exists on the anti-melanoma effects of fungal polysaccharides.
- Phellinus gilvus (PG) is a fungus with potential medicinal properties.
Purpose of the Study:
- To investigate the anti-melanoma effects of polysaccharides from Phellinus gilvus (PG).
- To evaluate the impact of PG on melanoma cell proliferation and apoptosis in vitro.
- To assess the in vivo efficacy of PG against melanoma in a mouse model.
Main Methods:
- In vitro studies using the B16F10 melanoma cell line.
- Sulforhodamine B (SRB) assay to determine cell proliferation.
- Sandwich enzyme-linked immunosorbent assay (ELISA) for apoptosis analysis.
- In vivo experiments using B16F10 melanoma cells allografted in athymic nude mice.
Main Results:
- PG significantly decreased B16F10 melanoma cell proliferation in a dose-dependent manner.
- PG markedly increased apoptosis in B16F10 melanoma cells in vitro.
- PG demonstrated significant inhibition of melanoma tumor growth in vivo.
- The anti-tumor effect in vivo correlated with an elevated melanoma apoptosis rate.
Conclusions:
- Phellinus gilvus (PG) polysaccharides exhibit potent anti-melanoma activity.
- PG effectively reduces melanoma cell proliferation and enhances apoptosis.
- PG shows promise as a natural therapeutic agent for melanoma treatment.
