Related Experiment Video
Updated: Aug 20, 2026

Wild-type Blocking PCR Combined with Sanger Sequencing for Detection of Low-frequency Somatic Mutation
Published on: August 23, 2024
[Use of PCR related methods in detection of gene mutation]
Zhi-Qiang Yan1, Sheng-Li Yang, Yi Gong
1Shanghai Research Center of Biotechnology, Institute of Biological Sciences, CAS, Shanghai 200233, China. zhiqiangyan@hotmail.com
Abstract:
Many inherited diseases and drug resistance have been attributed to mutations in corresponding genes. In this paper, several techniques based on PCR used in diagnosis were concluded. The development and research progress of Mismatch PCR were discussed in details. Some information about an assay that we developed for detection of antimicrobial resistance to quinolones was also described.
More Related Videos
10:41Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
Published on: March 29, 2017
08:23Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
Published on: September 25, 2018
Related Concept Videos
PCR - Polymerase Chain Reaction
PCR
Real Time RT-PCR
The real-time quantification of the number of amplified products is...